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phosphorylated smad family member 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated smad family member 3
    Phosphorylated Smad Family Member 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 3854 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated smad family member 3/product/Cell Signaling Technology Inc
    Average 98 stars, based on 3854 article reviews
    phosphorylated smad family member 3 - by Bioz Stars, 2026-02
    98/100 stars

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    phosphorylated smad family member 3  (Cell Signaling Technology Inc)
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    phosphorylated smad family member 3 p smad2 9520s  (Cell Signaling Technology Inc)
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    Effect of mitoxantrone on transforming growth factor-β (TGF-β)/SMAD signaling. (A) Fibroblasts were treated with mitoxantrone for 1 hour then stimulated with TGF-β for 1 hour. Phosphorylations of <t>SMAD2</t> and SMAD3 were determined by Western blot. Mitoxantrone slightly inhibited TGF-β-induced phosphorylation of SMAD3. (B) The protein levels of collagen type I α1 chain (COL1A1) and collagen type I α2 chain (COL1A2) were determined by Western blot. Mitoxantrone weakly but significantly inhibited TGF-β-induced collagen synthesis. Actin beta (ACTB) was used as a loading control.
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    phosphorylated smad family member 2/3 (p-smad2/3  (ABclonal Biotechnology)
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    Effect of mitoxantrone on transforming growth factor-β (TGF-β)/SMAD signaling. (A) Fibroblasts were treated with mitoxantrone for 1 hour then stimulated with TGF-β for 1 hour. Phosphorylations of <t>SMAD2</t> and SMAD3 were determined by Western blot. Mitoxantrone slightly inhibited TGF-β-induced phosphorylation of SMAD3. (B) The protein levels of collagen type I α1 chain (COL1A1) and collagen type I α2 chain (COL1A2) were determined by Western blot. Mitoxantrone weakly but significantly inhibited TGF-β-induced collagen synthesis. Actin beta (ACTB) was used as a loading control.
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    Effect of mitoxantrone on transforming growth factor-β (TGF-β)/SMAD signaling. (A) Fibroblasts were treated with mitoxantrone for 1 hour then stimulated with TGF-β for 1 hour. Phosphorylations of SMAD2 and SMAD3 were determined by Western blot. Mitoxantrone slightly inhibited TGF-β-induced phosphorylation of SMAD3. (B) The protein levels of collagen type I α1 chain (COL1A1) and collagen type I α2 chain (COL1A2) were determined by Western blot. Mitoxantrone weakly but significantly inhibited TGF-β-induced collagen synthesis. Actin beta (ACTB) was used as a loading control.

    Journal: Annals of Dermatology

    Article Title: Inhibitory Effect of Mitoxantrone on Collagen Synthesis in Dermal Fibroblasts

    doi: 10.5021/ad.2022.34.3.206

    Figure Lengend Snippet: Effect of mitoxantrone on transforming growth factor-β (TGF-β)/SMAD signaling. (A) Fibroblasts were treated with mitoxantrone for 1 hour then stimulated with TGF-β for 1 hour. Phosphorylations of SMAD2 and SMAD3 were determined by Western blot. Mitoxantrone slightly inhibited TGF-β-induced phosphorylation of SMAD3. (B) The protein levels of collagen type I α1 chain (COL1A1) and collagen type I α2 chain (COL1A2) were determined by Western blot. Mitoxantrone weakly but significantly inhibited TGF-β-induced collagen synthesis. Actin beta (ACTB) was used as a loading control.

    Article Snippet: The following primary antibodies were used in this study: COL1A1 (sc-8783), COL1A2 (sc-8786), HNRNPK (sc-28380), actin beta (ACTB) (sc-47778) (Santa Cruz Biotechnologies, Santa Cruz, CA, USA); LARP6 (H00055323-B01P; Abnova, Taipei, Taiwan); phosphorylated-SMAD family member 2 (p-SMAD2) (3108S), phosphorylated-SMAD family member 3 (p-SMAD2) (9520S) (Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Western Blot, Phospho-proteomics, Control